Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-B861-50	50 µg (0.1 mg/ml)	$460

PRKCA / PKC-Alpha Antibody - Anti-PRKCA / PKC-Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Thr497, IHC) LS‑B861

Name: IHC-plus™ PRKCA / PKC-Alpha Polyclonal Antibody Rabbit ELISA,IHC | LSBio
Brand: Lifespan BioSciences
SKU: LS-B861-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

Submit Review LSBio Guarantee

Note: This antibody replaces LS-C3383

Antibody:

PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pThr497) Antibody

Application:

IHC, IHC-P, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B861-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pThr497) Antibody

Application:

IHC, IHC-P, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

PKC-Alpha antibody LS-B861 is an unconjugated rabbit polyclonal antibody to human PKC-Alpha (PRKCA) (pThr497). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.

Target

Human PRKCA / PKC-Alpha

Synonyms

See All PRKCA / PKC-Alpha Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Synthetic peptide from human PRKCA / PKC-Alpha.

Epitope

pThr497

Specificity

Amino acids 491 to 501 with pTHR497 of human PRKCA

Applications

IHC
IHC - Paraffin (10 µg/ml)
ELISA (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B861 was determined to be 10 ug/ml.

Presentation

PBS, 0.1% Sodium Azide

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PRKCA / PKC-Alpha

Uniprot: P17252 NCBI: NM_002737 NP_002728.1

Validation

Anti-PRKCA / PKC-Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

See More About...

About LSBio Validation process