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Antibodies > PR / Progesterone Receptor > LS-C313405
 
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Catalog Number Size Price
LS-C313405-10 10 µg $318 
LS-C313405-100 100 µg $470 
PR / Progesterone Receptor Antibody - PGR/PR/Progesterone Receptor antibody. IHC(P): Human Intestinal Cancer Tissue.
PR / Progesterone Receptor Antibody - IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody. Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Progesterone Receptor Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PR / Progesterone Receptor Antibody - PGR/PR/Progesterone Receptor antibody. IHC(P): Human Lung Cancer Tissue.
PR / Progesterone Receptor Antibody - PGR/PR/Progesterone Receptor antibody. IHC(P): Human Breast Cancer Tissue.
PR / Progesterone Receptor Antibody - Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody at 0.1 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.
PR / Progesterone Receptor Antibody - WB of PGR/PR/Progesterone Receptor antibody. All lanes: Anti-Progesterone Receptor at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: SKOV Whole Cell Lysate at 40ug. Lane 4: Rat Brain Tissue Lysate at 40ug. Lane 5: Rat Testis Tissue Lysate at 40ug. Predicted bind size: 99KD. Observed bind size: 99KD.
PR / Progesterone Receptor Antibody - PGR/PR/Progesterone Receptor antibody. IHC(P): Human Intestinal Cancer Tissue.
PR / Progesterone Receptor Antibody - IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody. Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Progesterone Receptor Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PR / Progesterone Receptor Antibody - PGR/PR/Progesterone Receptor antibody. IHC(P): Human Lung Cancer Tissue.
PR / Progesterone Receptor Antibody - PGR/PR/Progesterone Receptor antibody. IHC(P): Human Breast Cancer Tissue.
PR / Progesterone Receptor Antibody - Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody at 0.1 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.
PR / Progesterone Receptor Antibody - WB of PGR/PR/Progesterone Receptor antibody. All lanes: Anti-Progesterone Receptor at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: SKOV Whole Cell Lysate at 40ug. Lane 4: Rat Brain Tissue Lysate at 40ug. Lane 5: Rat Testis Tissue Lysate at 40ug. Predicted bind size: 99KD. Observed bind size: 99KD.
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Polyclonal Rabbit anti‑Human PR / Progesterone Receptor Antibody (aa595‑933, IHC, WB) LS‑C313405

Polyclonal Rabbit anti‑Human PR / Progesterone Receptor Antibody (aa595‑933, IHC, WB) LS‑C313405

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Note: This antibody replaces LS-C389138
Antibody:
PR / Progesterone Receptor Rabbit anti-Human Polyclonal (aa595-933) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313405-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PR / Progesterone Receptor Rabbit anti-Human Polyclonal (aa595-933) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Progesterone Receptor antibody LS-C313405 is an unconjugated rabbit polyclonal antibody to Progesterone Receptor (PR) (aa595-933) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human PR / Progesterone Receptor
Synonyms
PGR | NR3C3 | PR | Progesterone receptor
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively.
Epitope
aa595-933
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PR / Progesterone Receptor
P06401 NM_000926 AAS00096.1

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Featured Products

PR / Progesterone Receptor Antibody - Anti-PGR / Progesterone Receptor antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
IHC‑plus™ PR / Progesterone Receptor Antibody (C‑Terminus) LS‑B2983
Species: Human
Applications: IHC, IHC - Paraffin
PR / Progesterone Receptor Antibody - PGR / Progesterone Receptor antibody Western blot of Mouse Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PR / Progesterone Receptor Antibody (aa201‑250) LS‑C116022
Species: Mouse
Applications: Western blot
Product
PR / Progesterone Receptor Antibody (clone Y85, Concentrated) LS‑C202898
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
PR / Progesterone Receptor Antibody - Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the -90k PR-A isoform and the -120 PR-13 isoform of the progesterone re.
PR / Progesterone Receptor Antibody (phospho‑Ser294) LS‑C216
Species: Human, Mouse, Rat
Applications: IHC, Western blot
PR / Progesterone Receptor Antibody - Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using Progesterone Receptor Antibody. The picture on the right is blocked with the synthesized peptide.
PR / Progesterone Receptor Antibody (aa261‑310) LS‑C117701
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
PR / Progesterone Receptor Antibody - IHC of Progesterone Receptor on FFPE Breast Carcinoma tissue.
PR / Progesterone Receptor Antibody LS‑C312154
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
See all PR / Progesterone Receptor Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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United States

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For technical assistance:
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