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Antibodies > PPP1R1B / DARPP-32 > LS-C801236
 
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Catalog Number Size Price
LS-C801236-100 100 µl (1 mg/ml) $379 
LS-C801236-200 200 µl (1 mg/ml) $421 
PPP1R1B / DARPP-32 Antibody - 1:100 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PPP1R1B / DARPP-32 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PPP1R1B / DARPP-32 Antibody - Staining MKN-45 cells treated with Forskolin 30 µM, 20 min by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PPP1R1B / DARPP-32 Antibody - Western blot analysis of DARPP-32 phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PPP1R1B / DARPP-32 Antibody - Western blot analysis of Phospho-DARPP-32 (Thr34) expression in various lysates
PPP1R1B / DARPP-32 Antibody - 1:100 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PPP1R1B / DARPP-32 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PPP1R1B / DARPP-32 Antibody - Staining MKN-45 cells treated with Forskolin 30 µM, 20 min by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PPP1R1B / DARPP-32 Antibody - Western blot analysis of DARPP-32 phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PPP1R1B / DARPP-32 Antibody - Western blot analysis of Phospho-DARPP-32 (Thr34) expression in various lysates
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Polyclonal Rabbit anti‑Human PPP1R1B / DARPP‑32 Antibody (phospho‑Thr34, IHC, IF, WB) LS‑C801236

Polyclonal Rabbit anti‑Human PPP1R1B / DARPP‑32 Antibody (phospho‑Thr34, IHC, IF, WB) LS‑C801236

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Antibody:
PPP1R1B / DARPP-32 Rabbit anti-Human Polyclonal (pThr34) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801236-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PPP1R1B / DARPP-32 Rabbit anti-Human Polyclonal (pThr34) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
DARPP-32 antibody LS-C801236 is an unconjugated rabbit polyclonal antibody to DARPP-32 (PPP1R1B) (pThr34) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PPP1R1B / DARPP-32
Synonyms
PPP1R1B | DARPP-32 | DARPP32
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human DARPP-32 around the phosphorylation site of Threonine 34.
Epitope
pThr34
Specificity
Phospho-DARPP-32 (Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Threonine 34.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP1R1B / DARPP-32
Q9UD71 NM_032192 NP_115568.2

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Featured Products

PPP1R1B / DARPP-32 Antibody - Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with l-Ptase.
PPP1R1B / DARPP‑32 Antibody (phospho‑Thr34) LS‑C25
Species: Rat, Human, Mouse, Bovine, Dog, Chicken
Applications: IHC, Western blot
Product
PPP1R1B / DARPP‑32 Antibody (aa51‑204) LS‑C36138
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PPP1R1B / DARPP-32 Antibody - Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical
PPP1R1B / DARPP‑32 Antibody (phospho‑Thr75) LS‑C27
Species: Rat, Human, Mouse, Bovine, Dog, Chicken
Applications: IHC, Western blot
Product
PPP1R1B / DARPP‑32 Antibody LS‑C22756
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, ELISA
PPP1R1B / DARPP-32 Antibody - Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PPP1R1B / DARPP‑32 Antibody (phospho‑Thr75) LS‑C358250
Species: Human, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, Western blot
PPP1R1B / DARPP-32 Antibody - Immunohistochemical analysis of DARPP32 (pT34) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PPP1R1B / DARPP‑32 Antibody (phospho‑Thr34) LS‑C358835
Species: Human, Mouse, Bovine, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all PPP1R1B / DARPP-32 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
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6737 Mowry Ave
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United States

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For technical assistance:
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