Human POMC / Proopiomelanocortin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Recombinant Monoclonal

r57

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

N-Terminus fragment of human ACTH conjugated to KLH. Antigen Molecular Weight: ACTH is ~5 kDa , and the POMC precursor is ~30 kDa . The molecular weight of POMC depends upon isoform variation and post-translational modifications.

N-Terminus

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH) - does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

• IHC - Paraffin (0.5 - 1 µg/ml)
• ELISA

ELISA - Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01189 NCBI: NM_000939 NP_000930.1