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Catalog Number Size Price
LS-C782672-100 100 µg (1 mg/ml) $574 
POLR2A / RNA polymerase II Antibody - IHC staining of FFPE human breast carcinoma with POLR2A antibody (clone CTD4H8). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
POLR2A / RNA polymerase II Antibody - Western blot testing of human HAP1, K562, PC3, HepG2, and mouse NIH3T3 lysate with POLR2A antibody. Routinely observed molecular weight: 200-250 kDa.
POLR2A / RNA polymerase II Antibody - SDS-PAGE analysis of purified, BSA-free POLR2A antibody (clone CTD4H8) as confirmation of integrity and purity.
POLR2A / RNA polymerase II Antibody - IHC staining of FFPE human breast carcinoma with POLR2A antibody (clone CTD4H8). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
POLR2A / RNA polymerase II Antibody - Western blot testing of human HAP1, K562, PC3, HepG2, and mouse NIH3T3 lysate with POLR2A antibody. Routinely observed molecular weight: 200-250 kDa.
POLR2A / RNA polymerase II Antibody - SDS-PAGE analysis of purified, BSA-free POLR2A antibody (clone CTD4H8) as confirmation of integrity and purity.
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Monoclonal Mouse anti‑Human POLR2A / RNA polymerase II Antibody (clone CTD4H8, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C782672

Monoclonal Mouse anti‑Human POLR2A / RNA polymerase II Antibody (clone CTD4H8, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C782672

Antibody:
POLR2A / RNA polymerase II Mouse anti-Human Monoclonal (Azide-free, BSA-free) (CTD4H8) Antibody
Application:
IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$574
LS-C782672-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
POLR2A / RNA polymerase II Mouse anti-Human Monoclonal (Azide-free, BSA-free) (CTD4H8) Antibody
Application:
IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
RNA polymerase II antibody LS-C782672 is an unconjugated mouse monoclonal antibody to RNA polymerase II (POLR2A) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A | HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
CTD4H8
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free. Also available Unmodified.
Immunogen
The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5.
Applications
  • IHC - Paraffin (1 - 2 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Optimal dilution of the recombinant POLR2A antibody should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Presentation
1X PBS
Storage
Aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR2A / RNA polymerase II
P24928 NM_000937 NP_000928.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2024