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| Catalog Number | Size | Price |
|---|---|---|
| LS-C26239-100 | 100 µl | $907 |
Monoclonal Mouse anti‑Human POLR2A / RNA polymerase II Antibody (IHC, WB) LS‑C26239
Monoclonal Mouse anti‑Human POLR2A / RNA polymerase II Antibody (IHC, WB) LS‑C26239
Antibody:
POLR2A / RNA polymerase II Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-Fr, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
POLR2A / RNA polymerase II Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-Fr, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect
Format:
Unconjugated, Unmodified
Specifications
Description
RNA polymerase II antibody LS-C26239 is an unconjugated mouse monoclonal antibody to RNA polymerase II (POLR2A) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A | HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
RNA polymerase II. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
Specificity
Excellent for detection of transcription activity, e. g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200kD band of non-phosphorylated and 240kD band of phosphorylated Pol IIA. Reactivities on Cultured Cell Lines (tested so far): Drosophila chromosomes, PTK-2, HeLa, Hep2. Epitope recognized: 794DDYGPESRGFVENSYLA822. Species cross-reactivity: Human, insects.
Applications
- IHC
- IHC - Frozen
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot (Western) and Immunohistochemistry (frozen tissue). Immunohistochemistry: 1:100 (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min). Incubation Time 1 hr at RT.
Presentation
0.09% Sodium Azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Restrictions
For research use only. Intended for use by laboratory professionals.
About POLR2A / RNA polymerase II
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse
Applications:
Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Dog, Zebrafish
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Hamster, Drosophila, Yeast, Plant
Applications:
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
Western blot, ELISA
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/26/2024
Date Requested: 11/26/2024
