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Catalog Number Size Price
LS-C674967-50 50 µg $294 
LS-C674967-100 100 µg (4.98 mg/ml) $360 
POLD1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
POLD1 Antibody - Western Blot Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate All lanes: POLD1 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa
POLD1 Antibody - Immunoprecipitating POLD1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of POLD1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLD1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
POLD1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
POLD1 Antibody - Western Blot Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate All lanes: POLD1 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa
POLD1 Antibody - Immunoprecipitating POLD1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of POLD1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLD1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
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Polyclonal Rabbit anti‑Human POLD1 Antibody (IHC, WB) LS‑C674967

Polyclonal Rabbit anti‑Human POLD1 Antibody (IHC, WB) LS‑C674967

Antibody:
POLD1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C674967-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
POLD1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
POLD1 antibody LS-C674967 is an unconjugated rabbit polyclonal antibody to human POLD1. Validated for IHC and WB.
Target
Human POLD1
Synonyms
POLD1 | CDC2 | CDC2 homolog | POLD
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human DNA polymerase delta catalytic subunit protein (1-123AA)
Applications
  • IHC - Paraffin (1:500 - 1:1000)
  • Western blot (1:500 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLD1
P28340 NM_002691 NP_002682.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024