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Catalog Number Size Price
LS-C801349-100 100 µl (1 mg/ml) $379 
LS-C801349-200 200 µl (1 mg/ml) $421 
POLA1 / DNA Polymerase Alpha 1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
POLA1 / DNA Polymerase Alpha 1 Antibody - Western blot analysis of DNA Polymerase a expression in 293 cells. The lane on the left is treated with the antigen-specific peptide.
POLA1 / DNA Polymerase Alpha 1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
POLA1 / DNA Polymerase Alpha 1 Antibody - Western blot analysis of DNA Polymerase a expression in 293 cells. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human POLA1 / DNA Polymerase Alpha 1 Antibody (IF, WB) LS‑C801349

Polyclonal Rabbit anti‑Human POLA1 / DNA Polymerase Alpha 1 Antibody (IF, WB) LS‑C801349

Antibody:
POLA1 / DNA Polymerase Alpha 1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801349-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
POLA1 / DNA Polymerase Alpha 1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Polymerase Alpha 1 antibody LS-C801349 is an unconjugated rabbit polyclonal antibody to human DNA Polymerase Alpha 1 (POLA1). Validated for IF, Peptide-ELISA and WB.
Target
Human POLA1 / DNA Polymerase Alpha 1
Synonyms
POLA1 | p180 | POLA | DNA Polymerase Alpha 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human DNA Polymerase alpha.
Specificity
DNA Polymerase alpha Antibody detects endogenous levels of total DNA Polymerase alpha.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLA1 / DNA Polymerase Alpha 1
P09884 NM_016937 NP_058633.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2024