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Antibodies > PML > LS-C352680
 
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Catalog Number Size Price
LS-C352680-100 100 µl (1 mg/ml) $299 
PML Antibody - Immunohistochemical analysis of PML staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PML Antibody - Immunofluorescent analysis of PML staining in COLO320 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PML Antibody - Western blot analysis of PML expression in K562 (A); COLO320 (B) whole cell lysates.
PML Antibody - Immunohistochemical analysis of PML staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PML Antibody - Immunofluorescent analysis of PML staining in COLO320 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PML Antibody - Western blot analysis of PML expression in K562 (A); COLO320 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human PML Antibody (N‑Terminus, IHC, IF, WB) LS‑C352680

Polyclonal Rabbit anti‑Human PML Antibody (N‑Terminus, IHC, IF, WB) LS‑C352680

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Antibody:
PML Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352680-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PML Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PML antibody LS-C352680 is an unconjugated rabbit polyclonal antibody to human PML (N-Terminus). Validated for ICC, IF, IHC and WB.
Target
Human PML
Synonyms
PML | PP8675 | Promyelocytic leukemia | Protein PML | TRIM19 | Promyelocytic leukemia protein | RING finger protein 71 | MYL | RNF71
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human PML.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of PML protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PML
P29590 NM_002675 NP_002666.1

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Species: Human
Applications: IHC, IHC - Paraffin, Western blot
PML Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
PML Antibody - Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10 ug/ml]
PML Antibody (clone 1D12) LS‑C197930
Species: Human, Rat
Applications: Immunofluorescence, Western blot, ELISA
See all PML Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/28/2024
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