Mouse PML

Mouse

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

6His fusion protein corresponding to aa 1-581 of mouse PML (promyelocytic leukemia protein).

aa1-581

Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not cross-react with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

• ICC
• Western blot (0.5 - 1 µg/ml)

Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-1 ug/ml of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P29590 NCBI: NM_002675 NP_002666.1