Human PMEL / SILV / gp100

Mouse

Human (tested or 100% immunogen sequence identity)

Rat, Dog

IgG1,k Monoclonal

SPM142

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Extract of pigmented melanoma metastases from lymph nodes.

PMEL / SILV / gp100

• IHC - Paraffin (0.5 - 1 µg/ml)

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. The optimal dilution of the gp100 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P40967 NCBI: NM_006928 NP_008859.1