Target
Human PLK1 / PLK-1
Synonyms
PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
A portion of GeneID: 5347 corresponding to amino acids between 325 and 375 of UniprotID: P53350
Specificity
Region between residues 325 and 375 of human Polo-like kinase using the numbering given in SwissProt entry P53350 (GeneID 5347).
Applications
- ICC (1:500 - 1:2000)
- Immunofluorescence (1:500 - 1:2000)
- Western blot (1:1000 - 1:10000)
- Immunoprecipitation (2 - 4 µg/mg lysate)
Usage
IHC-IF: 1:500-1:2000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee