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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1675-50 | 50 µg (0.5 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human PLK1 / PLK‑1 Antibody (clone 2A3, phospho‑Thr210, IHC, WB) LS‑B1675
IHC‑plus™ Monoclonal Mouse anti‑Human PLK1 / PLK‑1 Antibody (clone 2A3, phospho‑Thr210, IHC, WB) LS‑B1675
Note: This antibody replaces LS-C41059
Antibody:
PLK1 / PLK-1 Mouse anti-Human Monoclonal (pThr210) (2A3) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PLK1 / PLK-1 Mouse anti-Human Monoclonal (pThr210) (2A3) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PLK-1 antibody LS-B1675 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1) (pThr210). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PLK1 / PLK-1
Synonyms
PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
2A3
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human PLK1.
Epitope
pThr210
Specificity
Modified peptide
Applications
- IHC
- IHC - Paraffin (15 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1675 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1675 was determined to be 15 ug/ml.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced from tissue culture supernatant.
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PLK1 / PLK-1
Validation
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) for IHC, WB/Western LS-B1675 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Molli PR, Li DQ, Bagheri-Yarmand R, Pakala SB, Katayama H, Sen S, Iyer J, Chernoff J, Tsai MY, Nair SS, Kumar R. The Journal of cell biology. 2010 190:101-14.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/4/2024
Date Requested: 12/4/2024
