Target
Human PLCG1
Synonyms
PLCG1 | Phospholipase C, gamma 1 | Phospholipase C-148 | Phosphoinositidase C | Phospholipase C-II | Inositoltrisphosphohydrolase | PLC-gamma-1 | PLC1 | PLC-148 | PLC-II | PLC148 | NCKAP3 | Phospholipase C-gamma-1 | PLCgamma1
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1.
Specificity
Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not cross-react with phosphorylated PLC gamma 2 or other PLCs. Species cross-reactivity: Mouse. Species sequence Homology: Rat - 100%.
Applications
- Immunofluorescence
- Western blot (1:1000)
- Flow Cytometry (1:25)
- ELISA
Usage
Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee