Target
Human PLCG1
Synonyms
PLCG1 | Phospholipase C, gamma 1 | Phospholipase C-148 | Phosphoinositidase C | Phospholipase C-II | Inositoltrisphosphohydrolase | PLC-gamma-1 | PLC1 | PLC-148 | PLC-II | PLC148 | NCKAP3 | Phospholipase C-gamma-1 | PLCgamma1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide (KLH coupled) corresponding to the C-Terminus residues of human PLCgamma1.
Specificity
Recognizes endogenous levels of total human PLC gamma1 protein. Does not cross-react with PLCgamma2. Species cross-reactivity: mouse and rat. MW: 155kD.
Applications
- IHC
- IHC - Paraffin (1:25 - 1:100)
- Western blot (1:1000)
- Immunoprecipitation (1:50)
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Usage
Immunohistochemistry: LS-B2311 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2311 was determined to be 1:25-1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee