Target
Bovine PLCG1
Synonyms
PLCG1 | Phospholipase C, gamma 1 | Phospholipase C-148 | Phosphoinositidase C | Phospholipase C-II | Inositoltrisphosphohydrolase | PLC-gamma-1 | PLC1 | PLC-148 | PLC-II | PLC148 | NCKAP3 | Phospholipase C-gamma-1 | PLCgamma1
Reactivity
Bovine, Human, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Purified soluble phospholipase C (PLC) g-1 from bovine brain.
Specificity
Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.
Applications
- ICC (5 - 10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
- ELISA
Usage
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee