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Catalog Number Size Price
LS-C330883-20 20 µl $275 
LS-C330883-100 100 µl $389 
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded human prostate using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Western blot analysis of extracts of various cell lines, using PIK3CA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Immunohistochemistry of paraffin-embedded human prostate using PIK3CA antibodyat dilution of 1:100 (40x lens).
PIK3CA / PI3K Alpha Antibody - Western blot analysis of extracts of various cell lines, using PIK3CA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal Rabbit anti‑Human PIK3CA / PI3K Alpha Antibody (IHC, WB) LS‑C330883

Polyclonal Rabbit anti‑Human PIK3CA / PI3K Alpha Antibody (IHC, WB) LS‑C330883

Antibody:
PIK3CA / PI3K Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C330883-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PIK3CA / PI3K Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PI3K Alpha antibody LS-C330883 is an unconjugated rabbit polyclonal antibody to PI3K Alpha (PIK3CA) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human PIK3CA / PI3K Alpha
Synonyms
PIK3CA | MCAP | p110-alpha | p110alpha | PI3K | PI3-kinase p110 subunit alpha | PI3-kinase subunit alpha | PI3Kalpha | MCM | MCMTC | PI3K-alpha | PtdIns-3-kinase subunit alpha | CLOVE
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2). RLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME
Specificity
Human PIK3CA / PI3K
Applications
  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 124kDa, while the observed MW by Western blot was 115-120kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIK3CA / PI3K Alpha
P42336 NM_006218 NP_006209.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024