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Catalog Number Size Price
LS-B2451-100 100 µg $635 
PIK3CA / PI3K Alpha Antibody - Human Testis, Leydig Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
PIK3CA / PI3K Alpha Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
PIK3CA / PI3K Alpha Antibody - Anti-PIK3CA antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PIK3CA / PI3K Alpha Antibody - Human Testis, Leydig Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
PIK3CA / PI3K Alpha Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
PIK3CA / PI3K Alpha Antibody - Anti-PIK3CA antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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PathPlus™ Polyclonal Goat anti‑Human PIK3CA / PI3K Alpha Antibody (aa800‑1000, IHC) LS‑B2451

PathPlus™ Polyclonal Goat anti‑Human PIK3CA / PI3K Alpha Antibody (aa800‑1000, IHC) LS‑B2451

Note: This antibody replaces LS-C54785
Antibody:
PIK3CA / PI3K Alpha Goat anti-Human Polyclonal (aa800-1000) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$635
LS-B2451-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PIK3CA / PI3K Alpha Goat anti-Human Polyclonal (aa800-1000) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
PIK3CA (phosphatidylinositol 3-kinase catalytic subunit, PI3K) is the catalytic subunit of a heterodimeric lipid kinase that is a member of a family that regulates cell growth, transformation, adhesion, apoptosis, survival and motility. Activation of PIK3CA by a growth factor bound receptor tyrosine kinase and downstream production of PIP3 drives pathways that are involved in tumor development and progression in a variety of cancers. PIK3CA kinase is frequently mutated or amplified in cancer, and gene amplifications, deletions and more recently, somatic missense mutations in the PIK3CA gene have been reported in many human cancer types including cancers of the colon, breast, brain, liver, stomach, cervix and lung. Staining is expected to be primarily cytoplasmic but may also localize to cell membranes. PIK3CA is widely expressed in normal tissues. References: Br. J. Cancer 2006 94(4):455; Rosty, 2013; Chang, 2016; Xu, 2017; Oncogene 2000 19:2739
Target
Human PIK3CA / PI3K Alpha
Synonyms
PIK3CA | MCAP | p110-alpha | p110alpha | PI3K | PI3-kinase p110 subunit alpha | PI3-kinase subunit alpha | PI3Kalpha | MCM | MCMTC | PI3K-alpha | PtdIns-3-kinase subunit alpha | CLOVE
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide from the internal region of PIK3CA.
Epitope
aa800-1000
Specificity
Human PIK3CA.
Applications
  • IHC
  • IHC - Paraffin (2.5 - 5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2451 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2451 was determined to be 2.5-5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIK3CA / PI3K Alpha
P42336 NM_006218 NP_006209.2

Validation

PIK3CA / PI3K Alpha Antibody - Human Testis, Leydig Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Leydig Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Leydig Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)

PIK3CA / PI3K Alpha Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

PIK3CA / PI3K Alpha Antibody - Anti-PIK3CA antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIK3CA antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIK3CA antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ PIK3CA / PI3K Alpha Antibody (aa800-1000) for IHC LS-B2451 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/21/2024