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Catalog Number Size Price
LS-C313269-10 10 µg $318 
LS-C313269-100 100 µg (0.5 mg/ml) $470 
PFN2 / Profilin 2 Antibody - IHC analysis of PFN2 using anti-PFN2 antibody. PFN2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PFN2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PFN2 / Profilin 2 Antibody - Immunohistochemistry - Anti-Profilin 2 Antibody
PFN2 / Profilin 2 Antibody - Western blot analysis of PFN2 using anti-PFN2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PFN2 at approximately 15KD. The expected band size for PFN2 is at 15KD.
PFN2 / Profilin 2 Antibody - Western blot - Anti-Profilin 2 Antibody
PFN2 / Profilin 2 Antibody - Flow Cytometry analysis of A431 cells using anti-PFN2 antibody. Overlay histogram showing A431 cells stained with anti-PFN2 antibody(Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFN2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PFN2 / Profilin 2 Antibody - IHC analysis of PFN2 using anti-PFN2 antibody. PFN2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PFN2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PFN2 / Profilin 2 Antibody - Immunohistochemistry - Anti-Profilin 2 Antibody
PFN2 / Profilin 2 Antibody - Western blot analysis of PFN2 using anti-PFN2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PFN2 at approximately 15KD. The expected band size for PFN2 is at 15KD.
PFN2 / Profilin 2 Antibody - Western blot - Anti-Profilin 2 Antibody
PFN2 / Profilin 2 Antibody - Flow Cytometry analysis of A431 cells using anti-PFN2 antibody. Overlay histogram showing A431 cells stained with anti-PFN2 antibody(Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFN2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human PFN2 / Profilin 2 Antibody (aa125‑140, IHC, WB) LS‑C313269

Polyclonal Rabbit anti‑Human PFN2 / Profilin 2 Antibody (aa125‑140, IHC, WB) LS‑C313269

Note: This antibody replaces LS-C389126
Antibody:
PFN2 / Profilin 2 Rabbit anti-Human Polyclonal (aa125-140) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313269-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PFN2 / Profilin 2 Rabbit anti-Human Polyclonal (aa125-140) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
Profilin 2 antibody LS-C313269 is an unconjugated rabbit polyclonal antibody to Profilin 2 (PFN2) (aa125-140) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human PFN2 / Profilin 2
Synonyms
PFN2 | D3S1319E | PFL | Profilin II | Profilin-2 | Profilin 2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Profilin 2(125-140 aa NKKAYSMAKYLRDSGF), identical to the related mouse and rat sequences.
Epitope
aa125-140
Specificity
Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PFN2 / Profilin 2
P35080 NM_002628 NP_002619.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024