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Antibodies > PFN1 / Profilin 1 > LS-C757599
 
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Catalog Number Size Price
LS-C757599-200 200 µl $393 
PFN1 / Profilin 1 Antibody - All lanes: Anti-Profilin-1 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: 293 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PFN1 / Profilin 1 Antibody - Overlay histogram showing NIH/3T3 cells stained with Profilin-1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Profilin-1 Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PFN1 / Profilin 1 Antibody - Overlay histogram showing Hela cells stained with Profilin-1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Profilin-1 Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PFN1 / Profilin 1 Antibody - All lanes: Anti-Profilin-1 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: 293 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PFN1 / Profilin 1 Antibody - Overlay histogram showing NIH/3T3 cells stained with Profilin-1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Profilin-1 Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PFN1 / Profilin 1 Antibody - Overlay histogram showing Hela cells stained with Profilin-1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Profilin-1 Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human PFN1 / Profilin 1 Antibody (WB) LS‑C757599

Polyclonal Rabbit anti‑Human PFN1 / Profilin 1 Antibody (WB) LS‑C757599

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Antibody:
PFN1 / Profilin 1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757599-200
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PFN1 / Profilin 1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Profilin 1 antibody LS-C757599 is an unconjugated rabbit polyclonal antibody to Profilin 1 (PFN1) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow and WB.
Target
Human PFN1 / Profilin 1
Synonyms
PFN1 | ALS18 | Profilin-1 | Profilin 1 | Profilin I
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This Profilin-1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-140 amino acids from the human region of human Profilin-1.
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 15.1 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PFN1 / Profilin 1
P07737 NM_005022 NP_005013.1

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Featured Products

PFN1 / Profilin 1 Antibody - Western blot of recombinant PFN1 / Profilin 1. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PFN1 / Profilin 1 Antibody (aa1‑139, PE) LS‑C725289
Species: Human
Applications: Western blot
PFN1 / Profilin 1 Antibody - Western Blot Positive WB detected in Recombinant protein All lanes: Profilin antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa
PFN1 / Profilin 1 Antibody LS‑C500634
Species: Mercurialis annua
Applications: ELISA
PFN1 / Profilin 1 ELISA Kit
Human PFN1 / Profilin 1 (Sandwich ELISA) ELISA Kit - LS-F12421
Reactivity: Human
Range: 78-5000 pg/ml
PFN1 / Profilin 1 ELISA Kit
Human PFN1 / Profilin 1 (Sandwich ELISA) ELISA Kit - LS-F24009
Reactivity: Human
Range: 78.13-5000 pg/ml
PFN1 / Profilin 1 ELISA Kit
Rat PFN1 / Profilin 1 (Sandwich ELISA) ELISA Kit - LS-F19303
Reactivity: Rat
Range: 0.312-20 ng/ml
See all PFN1 / Profilin 1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025
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