Target
Rat PECAM-1 / CD31
Synonyms
PECAM1 | CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM-1 | GPIIA
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
R. Phycoerythrin.
Also available
Unconjugated or conjugated with
Biotin,
FITC.
Purification
Protein G purified
Immunogen
Activated, Lewis rat derived microglial cells
Specificity
Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid, pH7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.
Applications
- Flow Cytometry
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Use 1 ml of sterile Distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee