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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2364-50 | 50 µg (0.5 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human PECAM‑1 / CD31 Antibody (clone 2F7B2, IHC, WB) LS‑B2364
IHC‑plus™ Monoclonal Mouse anti‑Human PECAM‑1 / CD31 Antibody (clone 2F7B2, IHC, WB) LS‑B2364
Note: This antibody replaces LS-C47219
Antibody:
PECAM-1 / CD31 Mouse anti-Human Monoclonal (2F7B2) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PECAM-1 / CD31 Mouse anti-Human Monoclonal (2F7B2) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD31 antibody LS-B2364 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PECAM-1 / CD31
Synonyms
PECAM1 | CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM-1 | GPIIA
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
2F7B2
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant human PECAM1 / CD31.
Specificity
Ni-NTA purified recombinant CD31 expressed in E. coli strain BL21 (DE3).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2364 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2364 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.2, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PECAM-1 / CD31
Validation
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PECAM-1 / CD31 Antibody (clone 2F7B2) for IHC, WB/Western, ELISA LS-B2364 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Dog
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Rat, Rhesus monkey
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Mouse
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
