Target
Human PDPN / Podoplanin
Synonyms
PDPN | AGGRUS | HT1alpha-1 | Glycoprotein 36 | Glycoprotein, 36-KD | gp36 | gp38 | OTS8 | PA2.26 | PA2.26 antigen | Podoplanin | T1A | gp40 | HT1A-1 | HT1alpha-2 | p38 | T1-alpha | T1A-2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-EKVDGDTQTTVEKD, from the internal region of the protein sequence according to NP_006465.3NP_938203.2NP_001006625.1NP_001006626.1.
Specificity
Human PDPN / Podoplanin. This antibody is expected to recognize all isoforms (NP_006465.3; NP_938203.2; NP_001006625.1; NP_001006626.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Western Blot: Experiments gave bands at approx 25kD and 37kD in Human Brain (Cerebellum) lysates after 1 ug/ml antibody staining. The 37kD band corresponds to earlier findings in literature with different antibodies (Boucherot et al, Nephrol Dial Transplant. 2002 Jun;17(6):978-84; PMID: 12032185). This protein has a calculated MW of 24.9kD according to NP_006465.3. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee