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Vector Laboratories, Inc.
6737 Mowry Ave
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Catalog Number Size Price
LS-C800359-100 100 µl (1 mg/ml) $379 
LS-C800359-200 200 µl (1 mg/ml) $421 
PDGF-BB Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
PDGF-BB Antibody - Western blot analysis on NIH-3T3 cell lysates using PDGFB antibody. The lane on the left is treated with the antigen-specific peptide.
PDGF-BB Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
PDGF-BB Antibody - Western blot analysis on NIH-3T3 cell lysates using PDGFB antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PDGF‑BB Antibody (IF, WB) LS‑C800359

Polyclonal Rabbit anti‑Human PDGF‑BB Antibody (IF, WB) LS‑C800359

Antibody:
PDGF-BB Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800359-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PDGF-BB Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PDGF-BB antibody LS-C800359 is an unconjugated rabbit polyclonal antibody to PDGF-BB from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human PDGF-BB
Synonyms
PDGFB | PDGF-BB | PDGF-b | SSV | PDGF subunit B | PDGF-2 | PDGF2 | Proto-oncogene c-Sis | SIS | C-sis | PDGF, B chain
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PDGFB.
Specificity
PDGFB antibody detects endogenous levels of total PDGFB.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDGF-BB
P01127 NM_002608 NP_002599.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024