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Catalog Number Size Price
LS-B4182-50 50 µg (0.5 mg/ml) $485 
PDGF-BB Antibody - Anti-PDGFB antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PDGF-BB Antibody - Anti-PDGFB antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PDGF-BB Antibody - Goat Anti-PDGFB Antibody Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PDGF-BB Antibody - Goat Anti-PDGFB Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
PDGF-BB Antibody - Anti-PDGFB antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PDGF-BB Antibody - Anti-PDGFB antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PDGF-BB Antibody - Goat Anti-PDGFB Antibody Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PDGF-BB Antibody - Goat Anti-PDGFB Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human PDGF‑BB Antibody (aa222‑233, IHC, IF) LS‑B4182

IHC‑plus™ Polyclonal Goat anti‑Human PDGF‑BB Antibody (aa222‑233, IHC, IF) LS‑B4182

Note: This antibody replaces LS-C108405
Antibody:
PDGF-BB Goat anti-Human Polyclonal (aa222-233) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bovine, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B4182-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PDGF-BB Goat anti-Human Polyclonal (aa222-233) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bovine, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
PDGF-BB antibody LS-B4182 is an unconjugated goat polyclonal antibody to PDGF-BB (aa222-233) from human. It is reactive with human, bovine and sheep. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human PDGF-BB
Synonyms
PDGFB | PDGF-BB | PDGF-b | SSV | PDGF subunit B | PDGF-2 | PDGF2 | Proto-oncogene c-Sis | SIS | C-sis | PDGF, B chain
Host
Goat
Reactivity
Human, Bovine, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KHRKFKHTHDKT, from the C-Terminus of protein sequence according to NP_002599.1NP_148937.1.
Epitope
aa222-233
Specificity
Human PDGFB.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B4182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4182 was determined to be 3.75 ug/ml
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDGF-BB
P01127 NM_002608 NP_002599.1

Validation

PDGF-BB Antibody - Anti-PDGFB antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PDGFB antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PDGFB antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

PDGF-BB Antibody - Anti-PDGFB antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PDGFB antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PDGFB antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

IHC-plus™ PDGF-BB Antibody (aa222-233) for IHC, IF/Immunofluorescence LS-B4182 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and G alpha q-mediated Rho and alpha v beta 6 integrin-dependent activation of TGF- beta . Geng H, Lan R, Singha PK, Gilchrist A, Weinreb PH, Violette SM, Weinberg JM, Saikumar P, Venkatachalam MA. The American journal of pathology. 2012 Oct;181:1236-49.

Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease. Ana M Porras, Jennifer A Westlund, Austin D Evans, Kristyn S Masters. Proceedings of the National Academy of Sciences of the United States of America. 2018 January;115:E363-E371.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/23/2024