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Catalog Number	Size	Price
LS-C745005-100	100 µg (1 mg/ml)	$567

PARP1 Antibody - Immunohistochemistry with anti-PARP-1 antibody showing nuclear positivity in human lung tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-PARP-1 antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

PARP1 Antibody - Western Blot of recombinant PARP1 with rabbit anti-PARP1 (N-term ZF1) antibody. Lane 1: PARP1-Zinc Finger domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (N-term ZF1) antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP Gt-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 13 kDa for rPARP1 (N-term ZF1). Other band(s): none.

PARP1 Antibody - Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 ? per lane. Primary Antibody: Blot A: Anti-PARP1- n term; Blot B: Anti-PARP1- internal at 1?/mL for overnight at 4?. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4?. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40kDa in PARP1-AD only.

PARP1 Antibody - Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 (N-term ZF1) Antibody. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load: 5 µg per lane. Primary antibody: PARP1 (N-term ZF1) antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): none. Image in collaboration with Phil Lorenzi at MD Anderson.

PARP1 Antibody - Western Blot of rabbit anti-PARP1 N-term Antibody. Lane 1: Opal Pre-stained ladder Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 µg per lane. Primary antibody: PARP1 n-term antibody at 1ug/mL overnight at 4°C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody at 1:40,000 for 30 min at RT. Blocking Buffer: MB-073 for 30 min at RT. Predicted/Observed size: ~113 kDa for PARP1.

PARP1 Antibody - Western Blot of rabbit anti-PARP1 antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: PARP1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 113 kDa for PARP1.

PARP1 Antibody - Peggy Sue Size Separation Electropherogram of OVCAR-8 lysates in no-salt buffer and detected with Anti-PARP1 (N-term ZF1). UV immobilization time: 250 seconds. Protein concentration: 577 µg/mL; 120 s UV immobilization. Primary antibody concentration: 20µg/mL. Primary antibody incubation time: 180 min. Exposure time: multi-image analysis exposure. Predicted/observed: ~116 kDa.

PARP1 Antibody - OVCAR-8 Wild Type Lysate was separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the zinc finger 1 domain of PARP1. Primary Antibody: Anti-PARP1 (n-term) antibody 1:200 overnight at 4°C. Secondary Antibody: Goat anti-rabbit Peroxidase at 1:2,000 at RT for 30min. Blocking Buffer: BlockOut for 30 min at RT. Predicted/observed: ~110 kDa and pI 9.7.

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Polyclonal Rabbit anti‑Human PARP1 Antibody (N‑Terminus, IHC, WB) LS‑C745005

Name: Anti-PARP1 Antibody | Rabbit anti-Human Polyclonal IE,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C745005-100
Price: 567.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

PARP1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, WB, IE

Reactivity:

Human

Format:

Unconjugated, Unmodified

$567

LS-C745005-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PARP1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, WB, IE

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

PARP1 antibody LS-C745005 is an unconjugated rabbit polyclonal antibody to human PARP1 (N-Terminus). Validated for IE, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human PARP1

Synonyms

See All PARP1 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Unmodified

Immunogen

PARP1 (N-term ZF1) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with n-terminus region of human PARP1 zinc finger domain recombinant protein.

Epitope

N-Terminus

Specificity

This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.

Applications

IHC (1:100)
Western blot (1:1000)
Immunoelectrophoresis

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PARP1

Uniprot: P09874 NCBI: NM_001618 NP_001609.2

LSBio Ratings

PARP1 Antibody (N-Terminus) for IHC, WB/Western LS-C745005 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Mahmoud Labib, Zongjie Wang, Sharif U Ahmed, Reza M Mohamadi, Bill Duong, Brenda Green, Edward H Sargent, Shana O Kelley. Nature biomedical engineering. 2021 January;5:41-52.

Full Text Article PubMed: 32719513 PMC: PMC8436965

Submit a Publication for LS-C745005