Human PARK2 / Parkin 2

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

PRK109

Unconjugated

Tissue culture supernatant

Unmodified

Full length recombinant human Parkin.

C-Terminus

Reacts with the ~50 kD Parkin protein. Western blots of rat brain may also show a band of unknown origin at ~44 kD. Binds to the second RING domain (C-terminal region) of parkin protein. While the specific 50kD parkin signal was detected in Western blots of high salt fractions of wild-type mouse brain, no signal was detected in parkinnull mouse brain samples. Reactivity has been confirmed with recombinant human Parkin protein, human Parkin-transfected CHO cells, and human, mouse, and rat brain tissue homogenates. Species sequence Homology: the human Parkin sequence shares ~83% sequence homology with mouse and rat.

• Immunofluorescence (1:20 - 1:100)
• Western blot (1:20 - 1:100)
• Immunoprecipitation
• ELISA (1:100 - 1:1000)

Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunofluorescence. ELISA: 1:100-1:1000. Immunoprecipitation: 100 ul per test. Western Blot: 1:20-1:100. Immunofluorescence: 1:20-1:100.

0.1% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O60260 NCBI: NM_004562 NP_004553.2