Human Nuclear Antigen

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

NUCM1-1

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.

Human Nuclear Antigen

• IHC
• IHC - Paraffin (1:1)
• ICC
• Immunofluorescence

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. Titering of the Nuclear Antigen antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.