Human Nuclear Antigen

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Pig

IgG Recombinant Monoclonal

NM2984R

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Nuclei of HL60 cells.

This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

• IHC - Paraffin (0.25 - 0.5 µg/ml)

Immunohistology (Formalin-paraffin) (0.25-0.5 ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) - Optimal dilution for a specific application should be determined.

10 mM PBS

Aliquot and store at -20°Cto -80°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.