Target
Mouse NT5E / eNT / CD73
Synonyms
NT5E | 5-nucleotidase, ecto (CD73) | 5'-nucleotidase | 5 nucleotidase (CD73) | 5-NT | 5-nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 | CD73 antigen | E5NT | Ecto-5-nucleotidase | ENT | EN | NT | Purine 5-Prime-Nucleotidase | NT5 | NTE
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
CHO cell-derived rmCD73. (aa 29 - 549).
Specificity
Recognizes mouse CD73.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Flow Cytometry
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Direct ELISA: 0.5-1.0 ug/ml. Detection limit for rmCD73 is. ~0.3 ng/well. Western Blot: 0.1-0.2 ug/ml. Detection limit for rmCD73 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry (Murine splenocytes): 25 ug/ml. Add 100 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
40-50% glycerol, PBS
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee