Human NRSF / REST

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

GST fusion protein corresponding to residues 801-1097 of human REST (repressor element RE-1 silencing transcription factor). Also known as NRSF (neuronal restricted silencing factor).

aa801-1097

Recognizes REST, Mr 120-200kD. Species cross-reactivity: Human, mouse and rat.

• Western blot

Suitable for use in Western Blot. Western Blot Analysis: 0.5-2 ug/ml of this lot detected REST in nuclear extracts from HeLa and Raji cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-REST (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates REST.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13127 NCBI: NM_005612 NP_005603.3