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Catalog Number	Size	Price
LS-C756471-10	10 µg	$318
LS-C756471-100	100 µg	$470

NPPA / ANP Antibody - IHC analysis of ANP using anti-ANP antibody. ANP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ANP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

NPPA / ANP Antibody - IHC analysis of ANP using anti-ANP antibody. ANP was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-ANP Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

NPPA / ANP Antibody - IHC analysis of ANP using anti-ANP antibody. ANP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-ANP Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

NPPA / ANP Antibody - Western blot analysis of ANP using anti-ANP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANP antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ANP at approximately 17KD. The expected band size for ANP is at 17KD.

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Polyclonal Rabbit anti‑Mouse NPPA / ANP Antibody (IHC, WB) LS‑C756471

Name: Anti-NPPA / ANP Antibody | Rabbit anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756471-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

NPPA / ANP Rabbit anti-Mouse Polyclonal Antibody

Application:

IHC-P, WB, ELISA

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C756471-10

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

NPPA / ANP Rabbit anti-Mouse Polyclonal Antibody

Application:

IHC-P, WB, ELISA

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

ANP antibody LS-C756471 is an unconjugated rabbit polyclonal antibody to ANP (NPPA) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.

Target

Mouse NPPA / ANP

Synonyms

See All NPPA / ANP Antibodies

Host

Rabbit

Reactivity

Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived mouse ANP recombinant protein (Position: N25-R122).

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.