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Catalog Number	Size	Price
LS-C662921-100	100 µg	$470

NPC / NPC1 Antibody - Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates,Lane 2: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Niemann Pick C1 at approximately 170KD. The expected band size for Niemann Pick C1 is at 142KD.

NPC / NPC1 Antibody - Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody

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Polyclonal Rabbit anti‑Human NPC / NPC1 Antibody (WB) LS‑C662921

Name: Anti-NPC / NPC1 Antibody | Rabbit anti-Human Polyclonal ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662921-100
Price: 470.0000 USD
Availability: InStock

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Antibody:

NPC / NPC1 Rabbit anti-Human Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$470

LS-C662921-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

NPC / NPC1 Rabbit anti-Human Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NPC1 antibody LS-C662921 is an unconjugated rabbit polyclonal antibody to human NPC1 (NPC). Validated for ELISA and WB.

Target

Human NPC / NPC1

Synonyms

NPC1 | NPC | Niemann-Pick C1 protein | Niemann-Pick disease, type C1

See All NPC / NPC1 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

E. coli-derived human Niemann Pick C1 recombinant protein (Position: A1022-F1278).

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About NPC / NPC1

NCBI: NM_000271