Human NPC / NPC1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

17B5

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Fragments of Human NPC1 conjugated to a carrier protein.

Reacts with the human NPC1 protein. Species cross-reactivity: Confirmed with an NPC1 transfected I1061T cell line.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence (10 µg/ml)
• Western blot (1 - 2 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B3437 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3437 was determined to be 10 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000271