Human NOTCH2

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).

N-Terminus

This antiserum is directed against human NOTCH2. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:400 - 1:2000)
• ELISA (1:20000 - 1:100000)

Immunohistochemistry: LS-B719 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B719 was determined to be 1:500.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q04721 NCBI: NM_024408 NP_077719.2