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| Catalog Number | Size | Price |
|---|---|---|
| LS-C37725-100 | 100 µg | $786 |
Monoclonal Mouse anti‑Rat NOS1 / nNOS Antibody (N‑Terminus, IHC, WB) LS‑C37725
Monoclonal Mouse anti‑Rat NOS1 / nNOS Antibody (N‑Terminus, IHC, WB) LS‑C37725
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
NNOS antibody LS-C37725 is an unconjugated mouse monoclonal antibody to rat nNOS (NOS1) (N-Terminus). Validated for ELISA, IHC, IP and WB.
Target
Rat NOS1 / nNOS
Synonyms
NOS1 | BNOS | Constitutive NOS | IHPS1 | NNOS | NC-NOS | Nitric oxide synthase, brain | N-NOS | Neuronal NOS | NOS | NOS type I | Nos, type i
Host
Mouse
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant protein derived from the N-Terminus of rat nNOS protein.
Epitope
N-Terminus
Specificity
Specific for the ~160kD nNOS. Reactivity has been confirmed with rat brain tissue lysates. The nNOS target band at ~160kD was detected by immunoprecipitation and Western blots. Immunohistochemical tests using human, mouse, and rat brain tissue sections yielded positive and specific cytoplasmic staining in neurons and astrocytes. Based on amino acid sequence homology, the antibody is expected to recognize mouse (95%) and human (81%) nNOS.
Applications
- IHC
- IHC - Paraffin (2 µg/ml)
- Western blot (1 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Western Blot: 1 ug/ml. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 7 ug/each. Immunohistochemistry (FFPE): 2 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NOS1 / nNOS
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Featured Products
Species:
Human, Monkey, Mouse, Bat, Dog, Horse, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Guinea pig, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
