Human NKX3-1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

3-9

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Fusion protein containing the N-Terminus domain of the human Nkx3.1

N-Terminus

Reacts with human Nkx3.1 protein, and does not cross-react with mouse. On Western blots, this antibody detects a band at ~34kD. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• Western blot (1 - 3 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B3435 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3435 was determined to be 10 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99801 NCBI: NM_006167 NP_006158.2