Target
Human NKX2-5
Synonyms
NKX2-5 | Cardiac-specific homeo box | Cardiac-specific homeobox 1 | Cardiac-specific homeobox | CSX1 | CHNG5 | HLHS2 | NKX2E | NKX4-1 | NKX2.5 | Tinman paralog | VSD3 | CSX | Homeobox protein CSX | Homeobox protein Nkx-2.5 | NK2 homeobox 5
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1NP_001159647.1NP_001159648.1.
Specificity
Human NKX2-5. This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Immunofluorescence (10 µg/ml)
- Western blot (0.1 - 0.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kDa band observed in Human Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee