Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Purified, nitrated Keyhole Limpet Hemocyanin (KLH).
Specificity
Recognizes nitrated proteins from all species.
Applications
- ICC (5 - 10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunoprecipitation: To be determined by the researcher. Immunocytochemistry: 5-10 ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated A431 cells fixed with 95% ethanol/5% acetic acid.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee