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| Catalog Number | Size | Price |
|---|---|---|
| LS-C73595-100 | 100 µg | $890 |
Monoclonal Mouse Nitrotyrosine Antibody (IHC, WB) LS‑C73595
Monoclonal Mouse Nitrotyrosine Antibody (IHC, WB) LS‑C73595
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Nitrotyrosine antibody LS-C73595 is an unconjugated mouse monoclonal antibody to Nitrotyrosine. Validated for ELISA, IHC, IP and WB.
Host
Mouse
Clonality
IgG1
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Nitrotyrosine.
Specificity
Recognizes nitrotyrosine as both free amino acid and as present in nitrated proteins. Reactivity of this antibody with nitrated proteins is independent of the protein species of origin.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation
- ELISA (0.5 - 5 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 0.5-5 ug/ml. Western Blot: 1-2 ug/ml. Immunoprecipitation: ~5 ug/IP reaction. Immunohistochemistry (Frozen, paraffin): 1-2 ug/ml; Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Western Blot: Peroxynitrite treated A431 cells lysates.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Applications:
IHC, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
