Target
Human NGF
Synonyms
NGF | Beta-nerve growth factor | ProNGF | Beta-NGF | HSAN5 | NGFB
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Preservative Free
Immunogen
Recombinant Human NGF / NGFB protein
Specificity
Human NGF. Has cross-reactivity with Mouse ß-NGF in ELISA assay
Applications
- ELISA (2 µg/ml)
- Blocking (1 µg/ml)
- Neutralization (220 ng/ml)
Usage
Block: In a functional ELISA which immobilized recombinant Human ß-NGF at 2 ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human ß-NGF Monoclonal Antibody can block the binding of 1 ug/ml of biotinylated Human NTRK1 Protein to human ß-NGF, the EC50 is 0.90 ug/ml. Neutralization: The neutralization activity of ß-NGF Neutralizing Antibody is Measured by its ability to neutralize ß-NGF induced proliferation in the TF-1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 17-70 ng/mL in the presence of 220 ng/mL Recombinant Human ß-NGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee