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Antibodies > NF-L / NEFL > LS-C204554
 
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Catalog Number Size Price
LS-C204554-100 100 µl (1 mg/ml) $376 
NF-L / NEFL Antibody - Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCA-a-Int (red). These cells were mitotic but had several characteristics of neurons.
NF-L / NEFL Antibody - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).
NF-L / NEFL Antibody - Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCA-a-Int (red). These cells were mitotic but had several characteristics of neurons.
NF-L / NEFL Antibody - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).
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Monoclonal Mouse anti‑Pig NF‑L / NEFL Antibody (clone DA2, IF, WB) LS‑C204554

Monoclonal Mouse anti‑Pig NF‑L / NEFL Antibody (clone DA2, IF, WB) LS‑C204554

Submit Review LSBio Guarantee
Antibody:
NF-L / NEFL Mouse anti-Pig Monoclonal (DA2) Antibody
Application:
ICC, IF, WB
Reactivity:
Pig, Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204554-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NF-L / NEFL Mouse anti-Pig Monoclonal (DA2) Antibody
Application:
ICC, IF, WB
Reactivity:
Pig, Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
NEFL antibody LS-C204554 is an unconjugated mouse monoclonal antibody to NEFL (NF-L) from pig. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Pig NF-L / NEFL
Synonyms
NEFL | 68 kDa neurofilament protein | CMT1F | CMT2E | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
Host
Mouse
Reactivity
Pig, Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
DA2
Conjugations
Unconjugated
Purification
Purified from tissue culture supernatant.
Modifications
Unmodified
Immunogen
Preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H.
Specificity
Reacts with NF-L from human, cow, pig, mouse, rat and all other mammals, and also recognizes avian NF-L. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
Applications
  • ICC (1:500 - 1:1000)
  • Immunofluorescence (1:500 - 1:1000)
  • Western blot (1:5000)
Usage
For immunofluorescence, use NF-L / NEFL antibody diluted 1:500. For western blots try the antibody diluted 1:10000-1:20000.
Presentation
50% PBS, 50% glycerol plus 5mM NaN3
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NF-L / NEFL
P07196 NM_006158 NP_006149.2

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Featured Products

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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
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NF‑L / NEFL Antibody (clone OTI3G2) LS‑C175341
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
NF-L / NEFL Antibody - Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ NF‑L / NEFL Antibody LS‑B10376
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
NF‑L / NEFL Antibody (aa128‑271, Biotin) LS‑C678794
Species: Human
Applications: ELISA
NF-L / NEFL Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ NF‑L / NEFL Antibody (aa295‑344) LS‑B12562
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
NF-L / NEFL Antibody - Embryonic rat cortical cells grown in tissue culture and stained with NF-L / NEFL antibody (green) and the phosphorylated axonal form of neurofilament NF-H, using NF-H antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with NF-L / NEFL antibody but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.
NF‑L / NEFL Antibody (clone 7D1) LS‑C204557
Species: Human, Mouse, Rat, Bovine, Pig
Applications: ICC, Immunofluorescence, Western blot
See all NF-L / NEFL Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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