Human NF-L / NEFL

Mouse

Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

7D1

Unconjugated

Purified

Unmodified

Preparation of pig neurofilament NF-H and NF-L.

Reacts with NF-L from human, cow, pig, mouse, rat and all other mammals. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections. The epitope is not fully characterized since it could not be localized using 20 amino acid nested peptides but is known to be not within the a-helical region, amino acids 88-400 of the human protein.

• ICC (1:100 - 1:500)
• Immunofluorescence (1:100 - 1:500)
• Western blot (1:5000 - 1:10000)

For immunofluorescence, use NF-L / NEFL antibody diluted 1:100-1:500. For western blots try the antibody diluted 1:5000-1:10000.

50% PBS, 50% glycerol, plus 5 mM sodium azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07196 NCBI: NM_006158 NP_006149.2