Target
Human NCF2 / NOXA2 / p67phox
Synonyms
NCF2 | NADPH oxidase activator 2 | Neutrophil cytosol factor 2 | Neutrophil cytosolic factor 2 | NOXA2 | p67PHOX | NCF-2 | p67-PHOX
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
GST fusion protein corresponding to full-length human p67-phox.
Specificity
Recognizes human p67-phox at 65kD; a degradation product is detected at 45kD. Species cross-reactivity: Reported for bovine.
Applications
- ICC (1:500 - 1:4000)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:500-1:2000 detects p67-phox in RIPA lysates from HL-60 cells. Note: p67-phox is highly susceptible to proteases. Immunoprecipitation: 4 ul immunoprecipitated p67-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:500-1:4000 shows positive cytosolic immunostaining for p67-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Presentation
0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCF2 / NOXA2 / p67phox