Rat MYOG / Myogenin

Mouse

Rat, Human, Mouse, Dog, Pig (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

F5D

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.

aa138-158

Rat MYOG / Myogenin

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Western blot
• Immunoprecipitation
• Flow Cytometry
• ELISA

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor. The optimal dilution of the Myogenin antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15173 NCBI: NM_002479 NP_002470.2