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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1976-50 | 50 µg (0.5 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Rat MYOG / Myogenin Antibody (clone 5FD, aa30‑224, IHC, WB) LS‑B1976
IHC‑plus™ Monoclonal Mouse anti‑Rat MYOG / Myogenin Antibody (clone 5FD, aa30‑224, IHC, WB) LS‑B1976
Note: This antibody replaces LS-C570, LS-C73041
Antibody:
MYOG / Myogenin Mouse anti-Rat Monoclonal (aa30-224) (5FD) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
MYOG / Myogenin Mouse anti-Rat Monoclonal (aa30-224) (5FD) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
Myogenin antibody LS-B1976 is an unconjugated mouse monoclonal antibody to Myogenin (MYOG) (aa30-224) from rat. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Rat MYOG / Myogenin
Synonyms
MYOG | BHLHc3 | Myogenin | MYF4 | Myogenic factor 4 | Myf-4 | Myogenin (myogenic factor 4)
Host
Mouse
Reactivity
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Clone
5FD
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A recombinant GST fusion protein corresponding to amino acids 30-224 of rat myogenin.
Epitope
aa30-224
Specificity
The 5FD clone has been highly published; users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kD band should be observed. The can be used for gel shift assays.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen (5 - 10 µg/ml)
- ICC (5 µg/ml)
- Western blot (2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1976 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1976 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MYOG / Myogenin
Validation
Anti-Myogenin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Myogenin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MYOG / Myogenin Antibody (aa30-224, clone 5FD) for IHC, ICC, WB/Western LS-B1976 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Bovine, Goat, Horse, Pig, Rabbit, Sheep
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Western blot, ELISA, RNA interference
Species:
Human, Mouse, Rat, Pig
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Species:
Rat, Human, Mouse, Cat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
