Human MUC5B

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

5B19-2E

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide derived from the N-Terminus of the human MUC5B protein.

N-Terminus

Specific for the calculated ~590kD MUC5B protein. On Western Blots, recognizes a band significantly higher than 250kD. Reactivity has been confirmed with deglycosylated and secreted media from primary human tracheobranchial epithelial culture and a deglycosylated human saliva sample. Immunohistochemical assays were also performed using trachea samples from both normal and chronic obstructive pulmonary disease patients.

• IHC
• IHC - Paraffin
• Western blot (5 - 10 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 5-10 ug/ml. Immunohistochemistry (Formalin paraffin): 5-10 ug/ml. Note: Western Blot and Immunohistochemistry require deglycosylation. Refer to protocols.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9HC84 NCBI: NM_002458 NP_002449.2