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Catalog Number Size Price
LS-C313528-10 10 µg $318 
LS-C313528-100 100 µg (0.5 mg/ml) $470 
MUC5AC Antibody - IHC analysis of Mucin 5AC using anti-Mucin 5AC antibody. Mucin 5AC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Mucin 5AC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MUC5AC Antibody - WB of MUC5AC antibody. All lanes: Anti-MUC5AC at 0.5ug/ml. Lane 1: SGC Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: PANC Whole Cell Lysate at 40ug. Predicted bind size: 527KD. Observed bind size: 300KD.
MUC5AC Antibody - IHC analysis of Mucin 5AC using anti-Mucin 5AC antibody. Mucin 5AC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Mucin 5AC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MUC5AC Antibody - WB of MUC5AC antibody. All lanes: Anti-MUC5AC at 0.5ug/ml. Lane 1: SGC Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: PANC Whole Cell Lysate at 40ug. Predicted bind size: 527KD. Observed bind size: 300KD.
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Polyclonal Rabbit anti‑Human MUC5AC Antibody (aa552‑567, IHC, WB) LS‑C313528

Polyclonal Rabbit anti‑Human MUC5AC Antibody (aa552‑567, IHC, WB) LS‑C313528

Note: This antibody replaces LS-C388893
Antibody:
MUC5AC Rabbit anti-Human Polyclonal (aa552-567) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313528-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MUC5AC Rabbit anti-Human Polyclonal (aa552-567) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MUC5AC antibody LS-C313528 is an unconjugated rabbit polyclonal antibody to human MUC5AC (aa552-567). Validated for IHC and WB.
Target
Human MUC5AC
Synonyms
MUC5AC | Gastric mucin | Major airway glycoprotein | Muc5/5ac | Mucin-5AC | LeB | Lewis B blood group antigen | Tracheobronchial mucin | TBM | MUC-5AC | MUC5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human Mucin 5AC(552-567 aa TMQLFMQLAPKLRGQT).
Epitope
aa552-567
Specificity
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MUC5AC
P98088 AJ001403 CAA04738.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024