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Antibodies > MTOR > LS-C368624
 
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Catalog Number Size Price
LS-C368624-30 30 µl (1 mg/ml) $263 
LS-C368624-100 100 µl (1 mg/ml) $315 
LS-C368624-200 200 µl (1 mg/ml) $379 
MTOR Antibody - Immunohistochemical analysis of mTOR (pS2448) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MTOR Antibody - Immunofluorescent analysis of mTOR (pS2448) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MTOR Antibody - Western blot analysis of mTOR (pS2448) expression in HEK293T PMA-treated (A); HeLa (B); RAW264.7 PMA-treated (C); mouse liver (D) whole cell lysates.
MTOR Antibody - Immunohistochemical analysis of mTOR (pS2448) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MTOR Antibody - Immunofluorescent analysis of mTOR (pS2448) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MTOR Antibody - Western blot analysis of mTOR (pS2448) expression in HEK293T PMA-treated (A); HeLa (B); RAW264.7 PMA-treated (C); mouse liver (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human MTOR Antibody (pS2448, IHC, IF, WB) LS‑C368624

Polyclonal Rabbit anti‑Human MTOR Antibody (pS2448, IHC, IF, WB) LS‑C368624

Submit Review LSBio Guarantee
Antibody:
MTOR Rabbit anti-Human Polyclonal (pS2448) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C368624-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MTOR Rabbit anti-Human Polyclonal (pS2448) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
MTOR antibody LS-C368624 is an unconjugated rabbit polyclonal antibody to MTOR (pS2448) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human MTOR
Synonyms
MTOR | FRAP1 | FRAP2 | FRAP | Mammalian target of rapamycin | RAFT1 | Rapamycin and FKBP12 target 1 | RAPT1 | Rapamycin target protein 1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human mTOR.
Epitope
pS2448
Specificity
Recognizes endogenous levels of mTOR (pS2448) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MTOR
P42345 NM_004958 NP_004949.1

Publications (0)

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/30/2024
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