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| Catalog Number | Size | Price |
|---|---|---|
| LS-B650-50 | 50 µg (1 mg/ml) | $460 |
![MTOR Antibody - Anti-mTOR Antibody - Western Blot. Western blot of Affinity Purified anti-mTOR antibody shows detection of a band ~245 kD corresponding to human mTor (arrowhead). Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:650 for 2h at RT. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/pathplus-mtor-antibody-ls-b650/62918_192387.jpg)
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PathPlus™ Polyclonal Rabbit anti‑Human MTOR Antibody (IHC, WB) LS‑B650
PathPlus™ Polyclonal Rabbit anti‑Human MTOR Antibody (IHC, WB) LS‑B650
Note: This antibody replaces LS-C18805
Antibody:
MTOR Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
MTOR Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
MTOR (mechanistic target of rapamycin, FRAP1) is a serine/threonine protein kinase that acts as a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Overexpression of MTOR is related to tumorigenesis in breast, lung, bladder, brain, renal, thyroid, and prostate cancer as well as melanoma and leiomyosarcoma, and it is a target of therapeutic inhibitors. In immunohistochemistry of normal tissue, MTOR has membranous and cytoplasmic positivity in epithelial tissues throughout the body including intestinal crypts, the isthmic region of the stomach, pancreatic ductal cells, liver bile ducts, the kidney distal nephron and the bladder.
References: Dis Markers. 2017; 2017: 1397063, PMID: 28831205; Mod Pathol 2011;24:1553, PMID: 21822208; Mod Pathol 2012;25:197, PMID:22080063
Target
Human MTOR
Synonyms
MTOR | FRAP1 | FRAP2 | FRAP | Mammalian target of rapamycin | RAFT1 | Rapamycin and FKBP12 target 1 | RAPT1 | Rapamycin target protein 1
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat, Dog, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 2430-2460 of human mTOR.
Specificity
A BLAST analysis was used to suggest cross reactivity with mTOR protein from rat and mouse based on 100% homology with the immunizing sequence. Expect partial reactivity against mTOR homologues from zebrafish (94%) and dog (89%). Reactivity against homologues from other sources is not known.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:250 - 1:2000)
- ELISA (1:50000 - 1:100000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B650 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B650 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MTOR
Validation
Anti-MTOR antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MTOR antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MTOR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MTOR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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LSBio Ratings
PathPlus™ MTOR Antibody for IHC, WB/Western, ELISA LS-B650 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Dieckol Reduces Muscle Atrophy by Modulating Angiotensin Type II Type 1 Receptor and NADPH Oxidase in Spontaneously Hypertensive Rats. Seyeon Oh, Jin Young Yang, Chul Hyun Park, Kuk Hui Son, Kyunghee Byun. Antioxidants. 2021 Sep;10:1561.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/30/2024
Date Requested: 11/30/2024
